Mini Human 'Brains' Grown in a Dish

The first complete living model of the developing human brain has been created in a lab dish.

Researchers grew human stem cells in an environment that encouraged them to form pea-size gobs of brain tissue, which developed into distinct brain tissues, including a cerebral cortex and retina.

The minibrains were used to model microcephaly, a human genetic disorder in which brain size is dramatically reduced. Though not capable of consciousness or other higher cognitive functions, the minibrains allow scientists to study aspects of the developing human brain that are difficult to model in animals. [Inside the Brain: A Photo Journey Through Time]

A magnified image of a cerebral organoid showing cerebral tissue adjacent to developing retinal tissue (brown pigmented region). 

"The mouse brain is not always a good model system for the human brain," study researcher Jüergen Knoblich, of the Austrian Academy of Science's (IMBA) Institute of Molecular Biotechnology in Vienna, said at a news conference. "Our system allows us to study human-specific features of brain development."

Other groups have grown small pieces of neural tissue in the lab before, but none has been able to successfully grow tissue that contained both a cortex — the specialized outer layer of the brain — and other brain regions, Knoblich said. 

To create the minibrains, Knoblich and his team took human embryonic stem cells or induced pluripotent stem cells — both types of cells with a capacity to develop into any kind of tissue — and grew the cells in conditions that allowed them to form a tissue called neuroectoderm, which develops into the nervous system. The researchers embedded fragments of the tissue in droplets of gel to create a scaffold to guide further growth. They then transferred the droplets to a spinning bioreactor that increased nutrient absorption.

After 15 to 20 days, the tissue formed minibrains called cerebral organoids, each enclosing a fluid-filled region, much like the cerebral ventricles that contain cerebrospinal fluid in the human brain. After 20 to 30 days, some of the organoids formed defined brain areas, including a cerebral cortex (the brain's complex outer layer); retinal tissue, the light-sensitive part of the eye; meninges, the membranes that envelope the brain; and choroid plexus, which produces the cerebrospinal fluid that bathes the brain. [Infographic: See How the Mini Brain Grew in Lab]

The minibrains grew 2-3 millimeters (0.08-0.1 in) in diameter, and have survived in lab dishes for 10 months so far. Their size is limited, because they lack a circulatory system to provide nutrients and oxygen to their core regions. As a result, the brains could not develop the many layers seen in a real human brain, the researchers said.

In addition to modeling how a healthy human brain develops, the organoids can be used to model brain disorders. Knoblich and his colleagues used their minibrains to study microcephaly, a disorder not easily studied in mice because their brains are already smaller than human ones. They took skin cells from a microcephaly patient and reprogrammed them into stem cells, which they then grew into brain organoids.

The mini microcephaly brains grown from the patient's cells were smaller than ones grown from normal tissue, but had more neuron growth. The results suggest the brains of microcephaly patients develop neurons too early, before their brains have grown large enough. Other experiments showed the orientation in which the stem cells divide could also play a role in the disorder.

Biologist Gong Chen of Pennsylvania State University, who was not involved with the research, called it "a remarkable achievement," adding, "it opens the door for many studies on the human brain using human neurons."

This method of growing minibrains in a lab could be used to or test medications, or to study other brain disorders. "Ultimately, we would like to move to more common disorders, like schizophrenia and autism," Knoblich said, though he added that it's premature to speculate when scientists will develop minibrains complex enough to do so.

First Human Mind-Meld Created

Two new researchers have demonstrated the possibility of a human mind-meld, with one man's brain signals directing the other man's hand to move.

One man has controlled the movements of another person by sending brain signals via the Internet.

The demonstration is the first example of two human brains directly interacting.

"The Internet was a way to connect computers, and now it can be a way to connect brains," said University of Washington researcher Andrea Stocco, who participated in the experiment, in a statement. "We want to take the knowledge of a brain and transmit it directly from brain to brain."

The findings have not been published yet in a peer-reviewed journal.

The human-computer interface has grown by leaps and bounds in recent years. Scientists have developed methods for quadriplegics to move robotic limbs, grasping for chocolate or giving high-fives. Other researchers have created mind-melds between two rats, and between a rat and a human. 

On Aug. 12, University of Washington computer scientist Rajesh Rao donned a cap studded with electrodes that was attached to an electroencephalography machine. The cap read Rao's electrical brain activity.

In another room across campus, Stocco wore a swim cap fitted with a transcranial magnetic coil placed over the motor cortex, the brain region that controls movement. The magnetic coil delivered electrical signals into Stocco's brain.

As Rao played a video game and imagined moving his fingers to aim a cannon on the screen at a virtual target, his brain waves were sent to Stocco's brain via Internet. Stocco immediately noticed his hand involuntarily twitching, with his index finger aiming for the space bar, as if to press the button to fire the cannon.

"It was both exciting and eerie to watch an imagined action from my brain get translated into actual action by another brain," Rao said in a statement.

So far, the technology can only read simple signals and can't translate a person's complicated thought process. But the team hopes later iterations could become more complicated, perhaps aiding the disabled to translate their thoughts into actions.

"This was basically a one-way flow of information from my brain to his. The next step is having a more equitable two-way conversation directly between the two brains," Rao said.

Credit: University of Washington